Difference between revisions of "Part:BBa R0010"

Revision as of 21:33, 25 June 2006 (view source) Smelissali (Talk | contribs) ← Older edit		Revision as of 19:52, 23 July 2009 (view source) Meagan (Talk | contribs) Newer edit →
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	<partinfo>BBa_R0010 short</partinfo>		<partinfo>BBa_R0010 short</partinfo>
−	Inverting regulatory region controlled by LacI (<bbpart>BBa_C0010</bbpart>, <bbpart>BBa_C0011</bbpart>, etc.)	+	This part is an inverting regulator sensitive to LacI and CAP.
		+
		+	It contains two protein binding sites.  The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose.  The second binds LacI protein.
		+
		+
		+	* In the absence of LacI protein and CAP protein, this part promotes transcription.
		+	* In the presence of LacI protein and CAP protein, this part inhibits transcription.
		+	* LacI can be inhibited by [http://openwetware.org/wiki/IPTG IPTG].
		+	* LacI is coded by <bbpart>BBa_C0010</bbpart>
		+
		+
		+
		+	===Usage and Biology===
		+
		+	This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form.  It is the natural promoter for the LacZYA operon.
		+	It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.
		+
		+	<span class='h3bb'>Sequence and Features</span>
		+	<partinfo>BBa_R0010 SequenceAndFeatures</partinfo>
		+
		+
		+	<!-- Uncomment this to enable Functional Parameter display
		+	===Functional Parameters===
		+	<partinfo>BBa_R0010 parameters</partinfo>
		+	<!-- -->
−	The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bbpart>BBa_C0010</bbpart> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. <br>
−	In the presence of lactose or [http://openwetware.org/wiki/IPTG IPTG], an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bbpart>BBa_R0010</bbpart> to be used as a inverter or as a detector of lactose or [http://openwetware.org/wiki/IPTG IPTG].

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Revision as of 19:52, 23 July 2009

promoter (lacI regulated)

This part is an inverting regulator sensitive to LacI and CAP.

It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.

• In the absence of LacI protein and CAP protein, this part promotes transcription.
• In the presence of LacI protein and CAP protein, this part inhibits transcription.
• LacI can be inhibited by [http://openwetware.org/wiki/IPTG IPTG].
• LacI is coded by BBa_C0010

Usage and Biology

This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.

Sequence and Features

Usage and Biology

This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.

Sequence and Features