Difference between revisions of "Part:BBa K4370010:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
xxx
+
The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe).
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
xxx
+
The sequence has been synthetized.
  
 
===References===
 
===References===
 +
Bai et al., PNAS, 2015 - www.pnas.org/cgi/doi/10.1073/pnas.1511027112
 +
Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13
 +
Aubry et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00485-19

Revision as of 13:36, 18 August 2022


STREPTO_E.coli_CLONING_MODULE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe).


Source

The sequence has been synthetized.

References

Bai et al., PNAS, 2015 - www.pnas.org/cgi/doi/10.1073/pnas.1511027112 Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13 Aubry et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00485-19