Difference between revisions of "Part:BBa K4370010"
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<partinfo>BBa_K4370006 short</partinfo> | <partinfo>BBa_K4370006 short</partinfo> | ||
| − | This part corresponds to a cloning module allowing the cloning of gene of interest under the control of the Streptomyces constitutive strong promoter KasOP* between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). Between these two sites is inserted an expression cassette for the expression of mRFP1 (BBa_E1010) under the control of an <i> E. coli promoter <i/>. Therefore this module allows the easy red/white cloning of sequences of interest in place of the E. coli expression module. | + | This part corresponds to a cloning module allowing the cloning of gene of interest under the control of the Streptomyces constitutive strong promoter KasOP* between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). Between these two sites is inserted an expression cassette for the expression of mRFP1 (BBa_E1010) under the control of an <i> E. coli promoter <i/>. Therefore this module allows the easy red/white cloning of sequences of interest in place of the E. coli expression module. iol |
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Revision as of 11:13, 18 August 2022
KasOP*_promoter_STREPTO
This part corresponds to a cloning module allowing the cloning of gene of interest under the control of the Streptomyces constitutive strong promoter KasOP* between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). Between these two sites is inserted an expression cassette for the expression of mRFP1 (BBa_E1010) under the control of an E. coli promoter <i/>. Therefore this module allows the easy red/white cloning of sequences of interest in place of the E. coli expression module. iol
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]