Difference between revisions of "Part:BBa K4370005:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The sequence of the native gene STRBO_RS0123570 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces</i> genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i>. | + | The sequence of the native gene STRBO_RS0123570 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces</i> genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i>. For our project, the sequence has being cloned in pBAD33 by Golden Gate Assembly. |
Source | Source |
Revision as of 15:37, 17 August 2022
RBS_PRK_STRBO
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of the native gene STRBO_RS0123570 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli . For our project, the sequence has being cloned in pBAD33 by Golden Gate Assembly.
Source
Source
This sequence has been synthetized.