Difference between revisions of "Part:BBa K4370000:Design"

Line 13: Line 13:
 
===Source===
 
===Source===
  
This sequence has be synthetized.
+
This sequence has been synthetized.
  
 
===References===
 
===References===

Revision as of 14:42, 17 August 2022


cbbL_STRBO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 373


Design Notes

The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli .


Source

This sequence has been synthetized.

References