Difference between revisions of "Part:BBa K4150000"

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<partinfo>BBa_K4150000 short</partinfo>
 
<partinfo>BBa_K4150000 short</partinfo>
  
<span style="color:#00000000">This</span>RBS contains a g10 leader sequence from T7 bacteriophage gene 10.
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<span style="color:#00000000">This</span>RBS contains a g10 leader sequence from T7 bacteriophage gene 10, which encodes phage coat protein at very high expression level<ref>Olins PO, Devine CS, Rangwala SH, Kavka KS. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Gene. 1988 Dec 15;73(1):227-35. doi: 10.1016/0378-1119(88)90329-0. PMID: 3072257.</ref>.
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<ref>Kim S, Kim M, Ryu S. Development of an engineered bioluminescent reporter phage for the sensitive detection of viable Salmonella typhimurium. Anal Chem. 2014 Jun 17;86(12):5858-64. doi: 10.1021/ac500645c.</ref>
 
  
  

Revision as of 08:38, 11 August 2022


g10.RBS

ThisRBS contains a g10 leader sequence from T7 bacteriophage gene 10, which encodes phage coat protein at very high expression level[1].





Reference

  1. Olins PO, Devine CS, Rangwala SH, Kavka KS. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Gene. 1988 Dec 15;73(1):227-35. doi: 10.1016/0378-1119(88)90329-0. PMID: 3072257.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3