Difference between revisions of "Part:BBa K4268000:Design"

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*The sequence was edited to ensure that it is RCF10 and RCF1000 compatible.
 
*The sequence was edited to ensure that it is RCF10 and RCF1000 compatible.
  
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*The sequence contains SapI sites at the ends that are used in Level 0 Golden Gate Assembly.
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*To complete Level 0 Golden Gate Assembly, the sequence must contain SapI sites at the ends.
  
 
===Source===
 
===Source===

Revision as of 21:20, 26 July 2022


S-TIP37 Head-Tail Connector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 346
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The sequence was edited to ensure that it is RCF10 and RCF1000 compatible.
  • To complete Level 0 Golden Gate Assembly, the sequence must contain SapI sites at the ends.

Source

The source of this sequence is from the S-TIP37 genome (GenBanK: NC_048026.1)

References

The S-TIP37 genome was found on GenomeNet