Difference between revisions of "Part:BBa K4161002"
 
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JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli.
 
JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli.
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Source
 
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Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.
 
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The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4161002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4161002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4161002 parameters</partinfo>
 
<partinfo>BBa_K4161002 parameters</partinfo>
 
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===Source===
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The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)
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===Reference===
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Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.

Latest revision as of 12:10, 20 July 2022


JMK-H2

JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)

Reference

Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.