Difference between revisions of "Part:BBa K4475000"

Revision as of 23:58, 18 July 2022

CTB1 Gene from Cercospora beticola

This is the full exon only coding sequence for the CTB1 enzyme in the fungus cercospora beticola. The enzyme is the first in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin. FSU's iGEM team is looking to synthesize CTB1 in yeast as a proof of concept for cercosporin pathway expression in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The sequence has been codon optimized for Saccharomyces cerevisiae. An HA tag is embedded on the N-terminus for expression verification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3532
Illegal BamHI site found at 3441
Illegal BamHI site found at 5614
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6121
Illegal AgeI site found at 4195
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4475000 short</partinfo>
-This is the full exon only coding sequence for the CTB1 enzyme in the fungus cercospora beticola. The enzyme is the first in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin. FSU's iGEM team is looking to synthesize CTB1 in yeast as a proof of concept for cercosporin pathway expression in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The sequence has been codon optimized for Saccharomyces cerevisiae.
+This is the full exon only coding sequence for the CTB1 enzyme in the fungus cercospora beticola. The enzyme is the first in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin. FSU's iGEM team is looking to synthesize CTB1 in yeast as a proof of concept for cercosporin pathway expression in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The sequence has been codon optimized for Saccharomyces cerevisiae. An HA tag is embedded on the N-terminus for expression verification.
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