Difference between revisions of "Part:BBa K4347006"
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Sac7e is a DNA binding protien from the “7 kDa DNA-binding” family which is composed of chromatin proteins from the Sulfolobales archaeal order. Sac7e was isolated from thermoacidophilic archaeon <em> Sulfolobus acidocaldarius </em> and is structurally homologous to DNA binding protien Sso7d (https://parts.igem.org/Part:BBa_K3790002) isolated from thermophilic archaeon <em> Sulfolobus solfataricus </em>.[[Part:BBa_K4347006References|<sup>[1]</sup>]]  
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Sac7e is a DNA binding protien from the “7 kDa DNA-binding” family which is composed of chromatin proteins from the Sulfolobales archaeal order. Sac7e was isolated from thermoacidophilic archaeon <em> Sulfolobus acidocaldarius </em> and is structurally homologous to DNA binding protien Sso7d isolated from thermophilic archaeon <em> Sulfolobus solfataricus </em>.[[Part:BBa_K4347006References|<sup>[1]</sup>]]
  
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write small introduction about Sac7e here... eg, what is it? what organism does it come from?
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Structural homologue Sso7d from 2021 Fudan team: https://parts.igem.org/Part:BBa_K3790002
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 16:36, 18 July 2022

Sac7e

Sac7e is a DNA binding protien from the “7 kDa DNA-binding” family which is composed of chromatin proteins from the Sulfolobales archaeal order. Sac7e was isolated from thermoacidophilic archaeon Sulfolobus acidocaldarius and is structurally homologous to DNA binding protien Sso7d isolated from thermophilic archaeon Sulfolobus solfataricus .[1]

Structural homologue Sso7d from 2021 Fudan team: https://parts.igem.org/Part:BBa_K3790002

Usage and Biology

Write about usage and biology... examples used in previous work and difference between the other ssb protiens such as ss07d (part BBa K3790002)

Sac7e was isolated from Sulfolobus acidocaldarius, however Sso7d is a highly similar homolog from Sulfolobus solfataricus.

These proteins are hyper-thermostable and resistant to pH extremes. Sac7e is thermally stable to 85.5°C.

Compared to other similar proteins, Sac7e showed the highest affinity for dsDNA (KD = 11 μM), with binding sites ~ 6-8 bases per protein.

DNA binding protien "Sac7e" modelled in Pymol.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References


1. https://www.nature.com/articles/srep37274