Difference between revisions of "Part:BBa K4421030"

 
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An inducible caspase-9 suicide gene was placed under the control of a combined UAS-SV40 promoter. The GFP tag serves as a reporter and the 2A peptide sequence was intercalated between iCASP9 and the GFP tag to allow the fusion peptide chain to  separate into two peptide chains.  
 
An inducible caspase-9 suicide gene was placed under the control of a combined UAS-SV40 promoter. The GFP tag serves as a reporter and the 2A peptide sequence was intercalated between iCASP9 and the GFP tag to allow the fusion peptide chain to  separate into two peptide chains.  
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<div><ul>
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<center>
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  <li style="display: inline-block;">  [[File:iCASP9-2A-GFP.jpeg|thumb|none|250px|<b></b> The structure of CAR_library]]
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</li>
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</center>
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    </ul></div>
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4421030 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4421030 SequenceAndFeatures</partinfo>
  

Revision as of 06:41, 16 July 2022


pSV40-UAS-iCASP9-2A-GFP

An inducible caspase-9 suicide gene was placed under the control of a combined UAS-SV40 promoter. The GFP tag serves as a reporter and the 2A peptide sequence was intercalated between iCASP9 and the GFP tag to allow the fusion peptide chain to separate into two peptide chains.

  • The structure of CAR_library

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 781
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 463
    Illegal BsaI.rc site found at 1603
    Illegal BsaI.rc site found at 2514