Difference between revisions of "Part:BBa K4421027"

(Structure of CAR-library)
Line 5: Line 5:
 
As we have mentioned in <partinfo>BBa_K4421003</partinfo>, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on <partinfo>BBa_K4421003</partinfo>.
 
As we have mentioned in <partinfo>BBa_K4421003</partinfo>, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on <partinfo>BBa_K4421003</partinfo>.
  
===Structure of CAR-library===
+
===Structure of CAR_library===
 
Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ 2)transmembrane domain: CD8TM 3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all  
 
Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ 2)transmembrane domain: CD8TM 3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all  
 
kinds of scFvs which can be genetated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in <partinfo>BBa_K4421003</partinfo>.
 
kinds of scFvs which can be genetated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in <partinfo>BBa_K4421003</partinfo>.
 +
 
===Special Note===
 
===Special Note===
 
Since our part <partinfo>BBa_K4421003</partinfo> is a general concept without a specific sequence, we use <partinfo>BBa_K4421011</partinfo> cetuximab scfv to serve as a placeholder. In other words, the place where <partinfo>BBa_K4421011</partinfo> at can be replaced by any other scFv to form diverse CAR constructs.
 
Since our part <partinfo>BBa_K4421003</partinfo> is a general concept without a specific sequence, we use <partinfo>BBa_K4421011</partinfo> cetuximab scfv to serve as a placeholder. In other words, the place where <partinfo>BBa_K4421011</partinfo> at can be replaced by any other scFv to form diverse CAR constructs.

Revision as of 04:42, 16 July 2022


CAR_library

As we have mentioned in BBa_K4421003, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on BBa_K4421003.

Structure of CAR_library

Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ 2)transmembrane domain: CD8TM 3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all kinds of scFvs which can be genetated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in BBa_K4421003.

Special Note

Since our part BBa_K4421003 is a general concept without a specific sequence, we use BBa_K4421011 cetuximab scfv to serve as a placeholder. In other words, the place where BBa_K4421011 at can be replaced by any other scFv to form diverse CAR constructs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 1832
    Illegal NgoMIV site found at 1898
    Illegal NgoMIV site found at 2065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1439
    Illegal SapI.rc site found at 1996