Difference between revisions of "Part:BBa K4347007:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | How we came up with these residues to mutate. | |
− | + | Making point mutations in yasara - talking about the differences in gibbs free energy. | |
+ | ===Design Considerations=== | ||
+ | Considering which residues were in active site or not. Fingers are important for strand displacement and activity in taq(victor to fetch paper) and when mutated only 25% worked compared to thumb and palm domains. | ||
===Source=== | ===Source=== | ||
− | + | PDB: 6mu5 (insert link) | |
===References=== | ===References=== |
Revision as of 15:01, 8 July 2022
Bst with point mutations for enhanced thermal stability codon optimized for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 766
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
How we came up with these residues to mutate. Making point mutations in yasara - talking about the differences in gibbs free energy.
Design Considerations
Considering which residues were in active site or not. Fingers are important for strand displacement and activity in taq(victor to fetch paper) and when mutated only 25% worked compared to thumb and palm domains.
Source
PDB: 6mu5 (insert link)