Difference between revisions of "Part:BBa K176030:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K176030 short</partinfo> | <partinfo>BBa_K176030 short</partinfo> | ||
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Design: | Design: | ||
− | Input1 PoPS generate TetR | + | Input1 PoPS generate TetR-LVA |
pCon 0.70(J23101) generate LuxR | pCon 0.70(J23101) generate LuxR | ||
− | pLux/Tet(K176000) generate GFP, activated by LuxR and Input2 AHL, repressed by TetR and no aTc | + | pLux/Tet(K176000) generate GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc |
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===Source=== | ===Source=== |
Revision as of 14:00, 20 July 2009
Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 855
Illegal NheI site found at 878 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1833
Illegal BsaI.rc site found at 2577
Design Notes
Input1: PoPS
Input2: AHL
Output: GFP
Design:
Input1 PoPS generate TetR-LVA
pCon 0.70(J23101) generate LuxR
pLux/Tet(K176000) generate GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc
Source
K176006 K176026