Difference between revisions of "Part:BBa K176026:Design"

 
(→‎Design Notes)
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__NOTOC__
 
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<partinfo>BBa_K176026 short</partinfo>
 
<partinfo>BBa_K176026 short</partinfo>
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===Design Notes===
 
===Design Notes===
 +
 
Input: AHL
 
Input: AHL
 +
 
Output: GFP
 
Output: GFP
 +
 
Design:
 
Design:
 +
 
pCon 0.70(J23101) generate LuxR
 
pCon 0.70(J23101) generate LuxR
−
pLux/Tet(K176000) generate GFP, activated by LuxR and AHL, repressed by TetR and no aTc
 
−
 
  
 +
pLux/Tet(K176000) generate GFP, activated by LuxR and AHL, repressed by TetR and no aTc
  
 
===Source===
 
===Source===

Revision as of 13:22, 20 July 2009

TetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1729


Design Notes

Input: AHL

Output: GFP

Design:

pCon 0.70(J23101) generate LuxR

pLux/Tet(K176000) generate GFP, activated by LuxR and AHL, repressed by TetR and no aTc

Source

K176025 K176002

References