Difference between revisions of "Part:BBa J428100"

Revision as of 20:45, 8 June 2022

Interlab_NegC Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.

Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors

Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 71
Illegal NheI site found at 94
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Non-fluorescent negative control for InterLab studies 2022.
 It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR).
-It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpb_T1).
+It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1).
 It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.