Difference between revisions of "Part:BBa K259001"
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[http://ecoliwiki.net/colipedia/index.php/fiu:Gene_Product%28s%29#Domains.2FMotifs.2FModification_Sites E.Coli wiki net] | [http://ecoliwiki.net/colipedia/index.php/fiu:Gene_Product%28s%29#Domains.2FMotifs.2FModification_Sites E.Coli wiki net] | ||
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Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner) | Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner) | ||
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[http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Ecogene number EG13317] | [http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Ecogene number EG13317] | ||
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[http://www.uniprot.org/uniprot/P75780 UniProt Accession number P75780] | [http://www.uniprot.org/uniprot/P75780 UniProt Accession number P75780] | ||
Latest revision as of 17:35, 14 July 2009
fiu Outer Membrane Ferric Iron Transporter
Biology of Fiu
The fiu gene encodes for an outer membrane transporter found in E.coli, a gram negative bacterium. It is involved in the active transport (energy dependent uptake) of ferric iron from the extracellular environment. This outer membrane receptor can only bind ferric iron and is a catecholate siderophore. For uptake into the cell to be completed fiu has to complex with another protein known as TonB (a transperiplasmic protein) that will carry out the active transport by using ATP as an energy source.
What this Biobrick gives you upon expression
This biobrick encodes ONLY for fiu.
Fiu Statistics
Fiu belongs to the family of Ton-B dependent receptors and is related to fhuA, hence it also has a beta-barrel structure.
Chassis | Monomer/Multimer | Aminoacid Length/Weight | Localisation | Structure |
---|---|---|---|---|
E.Coli | Monomer | 760/81.96kDa | Outer Membrane/OMV | beta-barrel |
References
[http://ecoliwiki.net/colipedia/index.php/fiu:Gene_Product%28s%29#Domains.2FMotifs.2FModification_Sites E.Coli wiki net]
Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
[http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Ecogene number EG13317]
[http://www.uniprot.org/uniprot/P75780 UniProt Accession number P75780]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 682
Illegal AgeI site found at 820
Illegal AgeI site found at 1225
Illegal AgeI site found at 1999 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 724