Difference between revisions of "Part:BBa K4011996"

 
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<partinfo>BBa_K4011996 short</partinfo>
 
<partinfo>BBa_K4011996 short</partinfo>
  
 
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===Usage and Biology===
 
===Usage and Biology===
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sfkerrl
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==Design Considerations==
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cmgnsofgd
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==Characterization==
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tsodvndkg
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[[Image:T--LINKS China--Figure 16.png|thumbnail|750px|center|'''Figure 1:''' Construction and expression of TnaA-FMO proteins. A) Schematic representing TnaA-RBS-FMO, TnaA-Flexible linker-FMO, and TnaA-Rigid linker-FMO transformed into <i>E. coli DH5a ΔTnaA</i>. B. Schematic representing the structure of TnaA-linker-FMO. C) The sequences between TnaA and FMO, with the rbs and amino acids labelled. FL and RL stands for flexible linker and rigid linker respectively. D) SDS-PAGE analysis of TnaA-RBS-FMO, TnaA-RL-FMO, and TnaA-FL-FMO. ]]
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Revision as of 07:37, 29 March 2022

protein

abcabcabcabc

Usage and Biology

sfkerrl

Design Considerations

cmgnsofgd

Characterization

tsodvndkg

Figure 1: Construction and expression of TnaA-FMO proteins. A) Schematic representing TnaA-RBS-FMO, TnaA-Flexible linker-FMO, and TnaA-Rigid linker-FMO transformed into E. coli DH5a ΔTnaA. B. Schematic representing the structure of TnaA-linker-FMO. C) The sequences between TnaA and FMO, with the rbs and amino acids labelled. FL and RL stands for flexible linker and rigid linker respectively. D) SDS-PAGE analysis of TnaA-RBS-FMO, TnaA-RL-FMO, and TnaA-FL-FMO.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1219
  • 1000
    COMPATIBLE WITH RFC[1000]