Difference between revisions of "Part:BBa K3893006:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
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Design: rpoC Transcriptional terminator
+
We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
Source: rpoC Transcriptional terminator
+
It comes from a genetic reported sequence
  
 
===References===
 
===References===
 +
 +
Cambray, G., Guimaraes, J. C., Mutalik, V. K., Lam, C., Mai, Q. A., Thimmaiah, T., ... & Endy, D. (2013). Measurement and modeling of intrinsic transcription terminators. Nucleic acids research, 41(9), 5139-5148.

Latest revision as of 05:01, 18 December 2021


rpoC Transcriptional terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI

Source

It comes from a genetic reported sequence

References

Cambray, G., Guimaraes, J. C., Mutalik, V. K., Lam, C., Mai, Q. A., Thimmaiah, T., ... & Endy, D. (2013). Measurement and modeling of intrinsic transcription terminators. Nucleic acids research, 41(9), 5139-5148.