Difference between revisions of "Part:BBa K3893010:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None
 
  
 +
In order to use, test and produce the dsRNA we design before, we created a plasmid backbone with an insert that is able to receive different sequences of dsRNA with a BglII and KpnI digestion.
 +
 +
With this in mind, we face some shortcomings: first, it is a short fragment, with many sequence repeats, which makes it difficult for synthesis. Second, we want this insert to behave as a Biobrick compatible once it has the desired dsRNA sequence cloned inside.
  
  
 
===Source===
 
===Source===
  
−
By PCR using the cDNA of Fusarium oxysporum cubense RT1
+
Set of biobricks
−
 
+
−
 
+
−
===References===
+

Revision as of 04:10, 18 December 2021


Up part of cassette for dsRNA constitutive production 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 918
    Illegal SpeI site found at 164
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 834


Design Notes

In order to use, test and produce the dsRNA we design before, we created a plasmid backbone with an insert that is able to receive different sequences of dsRNA with a BglII and KpnI digestion.

With this in mind, we face some shortcomings: first, it is a short fragment, with many sequence repeats, which makes it difficult for synthesis. Second, we want this insert to behave as a Biobrick compatible once it has the desired dsRNA sequence cloned inside.


Source

Set of biobricks