Difference between revisions of "Part:BBa K3893008:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
aksjdnakjsdhasd
 
  
 +
In order to use, test and produce the dsRNA we design before, we created a plasmid backbone with an insert that is able to receive different sequences of dsRNA with a BglII and KpnI digestion.
  
 +
With this in mind, we face some shortcomings: first, it is a short fragment, with many sequence repeats, which makes it difficult for synthesis. Second, we want this insert to behave as a Biobrick compatible once it has the desired dsRNA sequence cloned inside.
  
−
===Source===
 
  
−
askdaksjdna
+
===Source===
  
−
===References===
+
Set of biobricks

Latest revision as of 04:09, 18 December 2021


Up part of cassette for dsRNA constitutive production 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 165
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 921
    Illegal SpeI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 165
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 837


Design Notes

In order to use, test and produce the dsRNA we design before, we created a plasmid backbone with an insert that is able to receive different sequences of dsRNA with a BglII and KpnI digestion.

With this in mind, we face some shortcomings: first, it is a short fragment, with many sequence repeats, which makes it difficult for synthesis. Second, we want this insert to behave as a Biobrick compatible once it has the desired dsRNA sequence cloned inside.


Source

Set of biobricks