Difference between revisions of "Part:BBa K3893001"

 
 
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<partinfo>BBa_K3893001 short</partinfo>
 
<partinfo>BBa_K3893001 short</partinfo>
  
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Description: dsRNA designed for the target gene Velvet
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This sequence is part of the [https://2021.igem.org/Team:Ecuador/Part_Collection Modular Platform for dsRNA production] in the proyect [https://2021.igem.org/Team:Ecuador/project AgroBactory 593].
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dsRNA designed to silence the Velvet target gene via RNAi mechanism.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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Once inside the pathogen, this dsRNA is further processed into RNA interference that can regulate the expression of any gene in the genome. Velvet gene is involved in the development of hyphae, the generation and germination of conidia, and the formation of virulence-inducing secondary metabolites. This has been a target gene to apply dsRNA against FOC R1 [1].
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3893001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3893001 SequenceAndFeatures</partinfo>
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===References===
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[1] S. B. Ghag, U. K. S. Shekhawat, and T. R. Ganapathi, “Host-induced post-transcriptional hairpin RNA-mediated gene silencing of vital fungal genes confers efficient resistance against Fusarium wilt in banana,” Plant Biotechnol J, vol. 12, no. 5, pp. 541–553, Jun. 2014, doi: 10.1111/pbi.12158.
  
  

Latest revision as of 03:35, 18 December 2021


dsRNA designed for the target gene Velvet

This sequence is part of the Modular Platform for dsRNA production in the proyect AgroBactory 593.

dsRNA designed to silence the Velvet target gene via RNAi mechanism.

Usage and Biology

Once inside the pathogen, this dsRNA is further processed into RNA interference that can regulate the expression of any gene in the genome. Velvet gene is involved in the development of hyphae, the generation and germination of conidia, and the formation of virulence-inducing secondary metabolites. This has been a target gene to apply dsRNA against FOC R1 [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] S. B. Ghag, U. K. S. Shekhawat, and T. R. Ganapathi, “Host-induced post-transcriptional hairpin RNA-mediated gene silencing of vital fungal genes confers efficient resistance against Fusarium wilt in banana,” Plant Biotechnol J, vol. 12, no. 5, pp. 541–553, Jun. 2014, doi: 10.1111/pbi.12158.