Difference between revisions of "Part:BBa K3893000:Design"

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===Design Notes===
 
===Design Notes===
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We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI
 
We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI
  
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===References===
 
===References===
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[1] A. Yurovsky, M. R. Amin, J. Gardin, Y. Chen, S. Skiena, y B. Futcher, “Prokaryotic coding regions have little if any specific depletion of Shine-Dalgarno motifs”, PLOS ONE, vol. 13, núm. 8, p. e0202768, ago. 2018, doi: 10.1371/journal.pone.0202768.
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Chen, H., Bjerknes, M., Kumar, R., & Jay, E. (1994). Determination of the optimal aligned spacing between the Shine–Dalgarno sequence and the translation initiation codon of Escherichia coli m RNAs. Nucleic acids research, 22(23), 4953-4957.

Latest revision as of 03:19, 18 December 2021


Shine Dalgarno for E coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI

Source

It comes from the original SD sequence reported for E.coli

References

Chen, H., Bjerknes, M., Kumar, R., & Jay, E. (1994). Determination of the optimal aligned spacing between the Shine–Dalgarno sequence and the translation initiation codon of Escherichia coli m RNAs. Nucleic acids research, 22(23), 4953-4957.