Difference between revisions of "Part:BBa K1682012:Experience"
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Utilizing the phosphate range of 0-100μM and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the [https://parts.igem.org/Part:BBa_K3725110 BBa_K3725110] phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels. | Utilizing the phosphate range of 0-100μM and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the [https://parts.igem.org/Part:BBa_K3725110 BBa_K3725110] phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels. | ||
| − | [[File:ASDJFCJBASJHBFD.png|thumb|center|800px|Figure | + | [[File:ASDJFCJBASJHBFD.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 150μM. ]] |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 20:37, 17 December 2021
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Lambert_GA 2021
Utilizing the phosphate range of 0-100μM and characterization protocol (See: Modified Protocol), we characterized the BBa_K3725110 phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.
User Reviews
UNIQ27b454d7d0698826-partinfo-00000000-QINU UNIQ27b454d7d0698826-partinfo-00000001-QINU