Difference between revisions of "Part:BBa K3725150"
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| − | In an attempt to increase the sensitivity of BBa_3725130 to extracellular phosphate, we designed part BBa_K3725150, a phosphate-sensitive biosensor with modified promoter BBa_K3725140 and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve. | + | In an attempt to increase the sensitivity of [https://parts.igem.org/Part:BBa_K3725130 BBa_3725130] to extracellular phosphate, we designed part BBa_K3725150, a phosphate-sensitive biosensor with modified promoter [https://parts.igem.org/Part:BBa_K3725140 BBa_K3725140] and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve. |
Latest revision as of 20:08, 17 December 2021
PhoB Redesigned Mutated Consensus Promoter w/ GFP Reporter
DESIGN
In an attempt to increase the sensitivity of BBa_3725130 to extracellular phosphate, we designed part BBa_K3725150, a phosphate-sensitive biosensor with modified promoter BBa_K3725140 and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1161