Difference between revisions of "Part:BBa K4060104"
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<partinfo>BBa_K4060104 short</partinfo>
 
<partinfo>BBa_K4060104 short</partinfo>
  
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Pre A1 (K4060100) ligated before TetR
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The gene fragment Pre B1 is composed of Pre A1 and the TetR gene(BBa_C0040). We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.
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This construct is our intermediate. For more information, please visit our results page
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Revision as of 14:26, 7 December 2021


[Pre B1] Pre A1 ligated before TetR

The gene fragment Pre B1 is composed of Pre A1 and the TetR gene(BBa_C0040). We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]