Difference between revisions of "Part:BBa K4060104:Experience"
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===Applications of BBa_K4060104=== | ===Applications of BBa_K4060104=== |
Revision as of 14:18, 7 December 2021
Assembly of Pre B1 (pSB1C3)
The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.
![](https://2021.igem.org/wiki/images/8/88/T--NYCU-Taipei--image9.png)
Fig.1 Agarose electrophoresis in order to verify Pre B1.
Applications of BBa_K4060104
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