Difference between revisions of "Part:BBa K4060101:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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====Assembly of Pre A2 (pSB1C3)====
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how you used this part and how it worked out.
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The gene fragment Pre A2 is composed of pTetR <partinfo>BBa_R0040</partinfo> and a double terminator <partinfo>BBa_B0015</partinfo>. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.
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<img src="https://2021.igem.org/wiki/images/3/35/T--NYCU-Taipei--0A45820D-B05E-44B2-BD88-998A183211BB.png" height="475px" width="750px"/>
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===Applications of BBa_K4060101===
 
===Applications of BBa_K4060101===

Revision as of 14:02, 7 December 2021


Assembly of Pre A2 (pSB1C3)

The gene fragment Pre A2 is composed of pTetR BBa_R0040 and a double terminator BBa_B0015. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.

Applications of BBa_K4060101

User Reviews

UNIQ10eae7242900381c-partinfo-00000003-QINU UNIQ10eae7242900381c-partinfo-00000004-QINU