Difference between revisions of "Part:BBa K4060112"

Revision as of 13:47, 7 December 2021

pJ102 is one of our tandem promoter design

K206000 + J23102

These tandem promoters are composed of the pBad(BBa_K206000) and a constitutive promoter with the RFP gene on the downstream. We digested the vector fragment BBa_K206000 with PstI and SpeI and the insert fragment constitutive promoter with RFP by XbaI and PstI. We ligated these fragments together to form pJ102, pJ118, pJ110, pJ116, and pJ113.

Agarose electrophoresis in order to verify the tandem promoters. Lane 1, 100bp ladder. Lane 2, pJ116 with BamHI and PstI. Lane 3, pJ118 with BamHI and PstI. Lane 4, pJ113 with BamHI and PstI. Lane 5, pJ110 with BamHI and PstI. Lane 6, pJ102 with BamHI and PstI.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
Illegal NheI site found at 145
Illegal NheI site found at 168
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 <div class="img">
-<img src="https://2021.igem.org/wiki/images/2/2b/T--NYCU-Taipei--pJ116PJ118PJ112PJ113PJ110.png" height=300px width = 500px"alt="">
+<img src="https://2021.igem.org/wiki/images/2/2b/T--NYCU-Taipei--pJ116PJ118PJ112PJ113PJ110.png" height=300px width = 100px"alt="">
 <div class="description">Agarose electrophoresis in order to verify the tandem promoters.
 Lane 1, 100bp ladder. Lane 2, pJ116 with BamHI and PstI. Lane 3, pJ118 with BamHI and PstI. Lane 4, pJ113 with BamHI and PstI. Lane 5, pJ110 with BamHI and PstI. Lane 6, pJ102 with BamHI and PstI.</div></div><br>