Difference between revisions of "Part:BBa K4060117"
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===Usage and Biology===
 
===Usage and Biology===
  
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There are some pBad tandem promoters whose RFP expression ability will drop as the concentration of L-arabinose increases such as pJ102, pJ118, pJ110. Here we take pJ102 for example. We can construct pJ102 before MazE while Pre C1 before MazF. Under this design, in general, the expression of MazE will be higher than MazF, since when the concentration of L-arabinose is zero, the RFP expression of pJ102 is better than that of Pre C1. When the L-arabinose concentration increases, the expression rate of pJ102 will drop while that of Pre C1 will increase simultaneously. As the MazF surpasses MazE, the killing system will turn on and achieve the kill switch.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4060117 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4060117 SequenceAndFeatures</partinfo>
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There are some pBad tandem promoters whose RFP expression ability will drop as the concentration of L-arabinose increases such as pJ102, pJ118, pJ110. Here we take pJ102 for example. We can construct pJ102 before MazE while Pre C1 before MazF. Under this design, in general, the expression of MazE will be higher than MazF, since when the concentration of L-arabinose is zero, the RFP expression of pJ102 is better than that of Pre C1. When the L-arabinose concentration increases, the expression rate of pJ102 will drop while that of Pre C1 will increase simultaneously. As the MazF surpasses MazE, the killing system will turn on and achieve the kill switch.
  
  

Revision as of 13:40, 7 December 2021


2021 NYCU Taipei final kill switch design

pJ102+thermometer RBS+MazE+Double terminator+Pre C1+Pre A4+Double terminator


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 245
    Illegal EcoRI site found at 873
    Illegal XbaI site found at 261
    Illegal XbaI site found at 889
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 245
    Illegal EcoRI site found at 873
    Illegal XbaI site found at 261
    Illegal XbaI site found at 889
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1232
  • 1000
    COMPATIBLE WITH RFC[1000]

There are some pBad tandem promoters whose RFP expression ability will drop as the concentration of L-arabinose increases such as pJ102, pJ118, pJ110. Here we take pJ102 for example. We can construct pJ102 before MazE while Pre C1 before MazF. Under this design, in general, the expression of MazE will be higher than MazF, since when the concentration of L-arabinose is zero, the RFP expression of pJ102 is better than that of Pre C1. When the L-arabinose concentration increases, the expression rate of pJ102 will drop while that of Pre C1 will increase simultaneously. As the MazF surpasses MazE, the killing system will turn on and achieve the kill switch.