Difference between revisions of "Part:BBa K233003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K233003 short</partinfo> | <partinfo>BBa_K233003 short</partinfo> | ||
− | + | This module can be used as an AND gate. | |
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+ | Input: Regulated Lac Repressor, Regulated LuxR protein | ||
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+ | Output: PoPs | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This construct functions as an AND gate. It is designed to work only when BOTH inputs are present. | ||
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+ | The LuxR promoter is activated only in the presence of LuxR-AHL complex, which binds to the promoter and activates transcription. | ||
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+ | The other part requires absence of the Lac Repressor protein. Lac repressor is inhibited in presence of lactose or IPTG. | ||
+ | Thus this GATE will be turned ON only when both LuxR-AHL complex AND Lactose (IPTG) are present. IF only LuxR-AHL complex is present, the Lac Repressor will remain bound to the binding site, preventing further transcription. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 09:35, 11 July 2009
AND Gate 1
This module can be used as an AND gate.
Input: Regulated Lac Repressor, Regulated LuxR protein
Output: PoPs
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]