Difference between revisions of "Part:BBa K4060103:Experience"
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We made a series of liquid cultures with different concentrations of L-arabinose. Then, we cultured <I>E. coli</I>  DH5-alpha with pBad and pBad-J23106 separately. After making the bacteria shake at 24°C for 24 hrs, we tested the RFP intensity and OD600 of these liquid cultures. | We made a series of liquid cultures with different concentrations of L-arabinose. Then, we cultured <I>E. coli</I>  DH5-alpha with pBad and pBad-J23106 separately. After making the bacteria shake at 24°C for 24 hrs, we tested the RFP intensity and OD600 of these liquid cultures. | ||
According to the figure, we can see that pBad-J23106 significantly outshines the original BioBrick_BBa_K206000. When the L-arabinose is lower than 0.025 M, both the pBad and pBad-J23106 promoters were induced. The RFP intensity of the improved part pBad-J23106 reached about 17000 at the L-arabinose concentration of 0.025, which is eight times higher than the original part pBad. The expression rate of pBad-J23106 still exceeds the origin part pBad at the concentration of L-arabinose higher than 0.025. We implemented this design in our kil-switch. Please visit our project result page for more information. | According to the figure, we can see that pBad-J23106 significantly outshines the original BioBrick_BBa_K206000. When the L-arabinose is lower than 0.025 M, both the pBad and pBad-J23106 promoters were induced. The RFP intensity of the improved part pBad-J23106 reached about 17000 at the L-arabinose concentration of 0.025, which is eight times higher than the original part pBad. The expression rate of pBad-J23106 still exceeds the origin part pBad at the concentration of L-arabinose higher than 0.025. We implemented this design in our kil-switch. Please visit our project result page for more information. | ||
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| + | <img src="https://2021.igem.org/wiki/images/e/e7/T--NYCU-Taipei--improvement_2.png" alt=""> | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 03:36, 22 October 2021
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4060103
We made a series of liquid cultures with different concentrations of L-arabinose. Then, we cultured E. coli DH5-alpha with pBad and pBad-J23106 separately. After making the bacteria shake at 24°C for 24 hrs, we tested the RFP intensity and OD600 of these liquid cultures. According to the figure, we can see that pBad-J23106 significantly outshines the original BioBrick_BBa_K206000. When the L-arabinose is lower than 0.025 M, both the pBad and pBad-J23106 promoters were induced. The RFP intensity of the improved part pBad-J23106 reached about 17000 at the L-arabinose concentration of 0.025, which is eight times higher than the original part pBad. The expression rate of pBad-J23106 still exceeds the origin part pBad at the concentration of L-arabinose higher than 0.025. We implemented this design in our kil-switch. Please visit our project result page for more information.
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User Reviews
UNIQ3ac7bf3ad1ed0f11-partinfo-00000000-QINU UNIQ3ac7bf3ad1ed0f11-partinfo-00000001-QINU