Difference between revisions of "Part:BBa K4060103"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4060103 short</partinfo> | <partinfo>BBa_K4060103 short</partinfo> | ||
| − | a | + | One of our goals is to create a better L-arabinose induced constitutive promoter. Here, we used a constitutive promoter (BioBrick_BBa_J23106) to co-regulate with pBad promoter (BBa_K206000). The pBad promoter is a positively regulated promoter, which will be inhibited by araC without the presence of L-arabinose. The L-arabinose can bind with araC and turn araC from a promoter inhibitor to a promoter inducer. |
| + | In our kill switch design, we hope to generate a stronger pBad promoter with greater expression rate and wider inducing range. With the regulation of both constitutive promoter and L-arabinose induced pBad promoter, we hope it can help the pBad to increase its expression ability. | ||
| + | In other words, we are trying to improve BioBrick_BBa_K206000. We want to ligate pBad promoter with a constitutive promoter to form a tandem promoter. We hypothesized that by adding a constitutive promoter beside pBad, the expression of the tandem promoter will be better than the normal pBad promoter (BioBrick_BBa_K206000). We compared the intensity of RFP gene constructed behind the pBad promoter and pBad-J23106 tandem promoter to see if the tandem promoter has better expression rate. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 03:31, 22 October 2021
[Pre A4] a pBad ligated before J23106
One of our goals is to create a better L-arabinose induced constitutive promoter. Here, we used a constitutive promoter (BioBrick_BBa_J23106) to co-regulate with pBad promoter (BBa_K206000). The pBad promoter is a positively regulated promoter, which will be inhibited by araC without the presence of L-arabinose. The L-arabinose can bind with araC and turn araC from a promoter inhibitor to a promoter inducer. In our kill switch design, we hope to generate a stronger pBad promoter with greater expression rate and wider inducing range. With the regulation of both constitutive promoter and L-arabinose induced pBad promoter, we hope it can help the pBad to increase its expression ability. In other words, we are trying to improve BioBrick_BBa_K206000. We want to ligate pBad promoter with a constitutive promoter to form a tandem promoter. We hypothesized that by adding a constitutive promoter beside pBad, the expression of the tandem promoter will be better than the normal pBad promoter (BioBrick_BBa_K206000). We compared the intensity of RFP gene constructed behind the pBad promoter and pBad-J23106 tandem promoter to see if the tandem promoter has better expression rate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 145
Illegal NheI site found at 168 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]