Difference between revisions of "Part:BBa K3945002"
(→Usage and Biology) |
|||
| Line 5: | Line 5: | ||
__TOC__ | __TOC__ | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <p>Lanmodulin is recently a novel lanthanide binding protein from <I>Methylobacterium extorquens</i> that displays more than 100-million fold selectivity for rare earth elements [1]. Such affinity and selectivity have not been observed in any previously studied macromolecule. In addition, lanmodulin is extremely robust, capable of withstanding temperatures as high as 95 °C and pH levels as low as 2.5 [2]. Thus, allowing it to be the perfect molecular tool for us in an efficient rare earth recovery system.</p> | + | <p>Lanmodulin is recently a novel lanthanide binding protein from <I>Methylobacterium extorquens</i> that displays more than 100-million fold selectivity for rare earth elements [1]. Such affinity and selectivity have not been observed in any previously studied macromolecule. In addition, lanmodulin is extremely robust, capable of withstanding temperatures as high as 95 °C and pH levels as low as 2.5 [2]. Thus, allowing it to be the perfect molecular tool for us in an efficient rare earth recovery system.</p> |
| + | |||
| + | <p> We have mutated the lanmodulin sequence using Family Conservation Algorithm to improve the binding capacity of lanmodulin's fourth binding pocket. </p> | ||
===Design=== | ===Design=== | ||
| − | + | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 03:30, 22 October 2021
FC-Mutated Lanmodulin (FC LanM)
Usage and Biology
Lanmodulin is recently a novel lanthanide binding protein from Methylobacterium extorquens that displays more than 100-million fold selectivity for rare earth elements [1]. Such affinity and selectivity have not been observed in any previously studied macromolecule. In addition, lanmodulin is extremely robust, capable of withstanding temperatures as high as 95 °C and pH levels as low as 2.5 [2]. Thus, allowing it to be the perfect molecular tool for us in an efficient rare earth recovery system.
We have mutated the lanmodulin sequence using Family Conservation Algorithm to improve the binding capacity of lanmodulin's fourth binding pocket.
Design
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 111
Illegal BamHI site found at 186 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]