Difference between revisions of "Part:BBa K3738007:Design"

Latest revision as of 03:29, 22 October 2021

MS2 Bacteriophage Capsid Protein with N-Terminal Polyarginine Tag and C-Terminal Histidine Tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 193
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for use in E.coli.

This part is an improved version of the part BBa_K2683012, and has been modified to a C-terminal Histidine Tag in addition to the N-terminal polyarginine tag. This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 bacteriophage.

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

@@ Line 9: / Line 9: @@
 This part has been codon optimized for use in <i>E.coli</i>.
-This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal polyarginine tag and a C-terminal Histidine Tag.
+This part is an improved version of the part BBa_K2683012, and has been modified to a C-terminal Histidine Tag in addition to the N-terminal polyarginine tag.
 This part has been codon optimized for use in E.coli.
 ===Source===