Difference between revisions of "Part:BBa K3738006:Design"

(References)
(Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.
+
This part is an improved version of the part BBa_K2683012, and has been modified to include an C-terminal histidine tag and has had the polyarginine tag removed.
 
This part has been codon optimized for use in E.coli.
 
This part has been codon optimized for use in E.coli.
  

Latest revision as of 03:27, 22 October 2021


MS2 Bacteriophage Capsid Protein with C-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an improved version of the part BBa_K2683012, and has been modified to include an C-terminal histidine tag and has had the polyarginine tag removed. This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 bacteriophage.

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664

[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

https://www.uniprot.org/uniprot/P03612