Difference between revisions of "Part:BBa K3745043"

Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
 +
<html>
 
<partinfo>BBa_K3745043 short</partinfo>
 
<partinfo>BBa_K3745043 short</partinfo>
  
Line 8: Line 9:
  
 
<figure>
 
<figure>
<img src="https://2021.igem.org/wiki/images/3/3f/T--BNDS_China--HPLC-MS_result.png" width="800" height="auto"/>
+
<p style="text-align:center;"><img src="https://2021.igem.org/wiki/images/3/3f/T--BNDS_China--HPLC-MS_result.png" width="800" height="auto"/>
 
<figcaption>
 
<figcaption>
 
<p style="text-align:center;"><b>Figure 1.Result of HPLC-MS measurement of IPTG induced culture</b></p>
 
<p style="text-align:center;"><b>Figure 1.Result of HPLC-MS measurement of IPTG induced culture</b></p>
Line 27: Line 28:
 
<partinfo>BBa_K3745043 parameters</partinfo>
 
<partinfo>BBa_K3745043 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
</html>

Revision as of 03:24, 22 October 2021


BBa_K3745043 short The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli. With the help of this plasmid and espacially rhlA, B, and C, three major materials can compound to form rhamnolipids: Glycolysis or Gluconeogenesis, ß-oxidation, and Fatty acid. To be specific, 3-hydroxyacyl-CoA that is derived from ß-oxidation can compound with 3-hydroxyacyl-ACP under a special enzyme——RhlA to form 3-hydroxyalanoyl-3hydroxyalkanoyl(HAA)-ACP or S-CoA. Eventually, dTDP-L-rhamnose and HAA will combine together under the catalytic effects of RhlB to forge Mono-rhamnolipids. And then, with the assistance of RhlC, Mono-rhamnolipids can be turned into Di-rhamnolipids which are experimentally proved to be a better biosurfactant than mono-rhamnolipid. It is worth informing that the Plac in the DNA feature's graph is a reverse promoter (activate LacI instead of activating rhlA, B, and C). The arrow on the feature is not accurate due to the reason that the image of the promoter won't appear in the reverse direction. Nevertheless, the DNA sequence is posted in the correct arrangement which Plac is responsible for activating LacI.

Figure 1.Result of HPLC-MS measurement of IPTG induced culture

Sequence and Features BBa_K3745043 SequenceAndFeatures