Difference between revisions of "Part:BBa K3738007:Design"
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This part has been codon optimized for use in <i>E.coli</i>.
 
This part has been codon optimized for use in <i>E.coli</i>.
  
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This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.
+
This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal polyarginine tag and a C-terminal Histidine Tag.  
 
This part has been codon optimized for use in E.coli.
 
This part has been codon optimized for use in E.coli.
  
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===References===
 
===References===
  
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[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
+
[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664 <br>
 
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138
 
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

Revision as of 03:24, 22 October 2021


MS2 Bacteriophage Capsid Protein with N-Terminal Polyarginine Tag and C-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for use in E.coli.

This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal polyarginine tag and a C-terminal Histidine Tag. This part has been codon optimized for use in E.coli.


Source

This part comes from the MS2 bacteriophage.

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138