Difference between revisions of "Part:BBa K1060000"
(→‎2021 XJTU-China's Improvement)
Line 19: Line 19:
  
 
==2021 XJTU-China's Improvement==
 
==2021 XJTU-China's Improvement==
 +
This part has been improved by 2021 team: XJTU-China. The improved version, AroG-S211F([https://parts.igem.org/Part:BBa_K3832000 BBa_K3832000], has been analyzed by structure modelling and measured.
 +
<br>
 +
Here some important results of our improvement have been shown below. And for more detailed methods and results, please check pages of '''[https://parts.igem.org/Part:BBa_K3832000 Part:BBa_K3832000]''' and our wiki '''[https://2021.igem.org/Team:XJTU-China/Improve Team:XJTU-China/Improve]'''
 +
 +
===Structural Modelling Result===
 +
===Characterization & Measurement===

Revision as of 03:19, 22 October 2021

AroG; DAHP synthase

The aroG gene, coding for 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, is a part of the Shikimate pathway. It converts phosphoenolpyruvate and erythrose-4P to chorismate. In our project chorismate was one of the precursors needed for the biosynthesis of methyl salicylate (see also BBa_K1060003). As basic chorismate levels inside E. coli are limited. We wanted to upregulate the biosynthesis of chorismate. The basic aroG gene can be repressed by phenylalanine. However, mutatations in the amino acid sequence (Pro150Leu and Leu175Asp) can prevent this repression by phenylalanine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 241



2021 XJTU-China's Improvement

This part has been improved by 2021 team: XJTU-China. The improved version, AroG-S211F(BBa_K3832000, has been analyzed by structure modelling and measured.
Here some important results of our improvement have been shown below. And for more detailed methods and results, please check pages of Part:BBa_K3832000 and our wiki Team:XJTU-China/Improve

Structural Modelling Result

Characterization & Measurement