Difference between revisions of "Part:BBa K3771022"

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	<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>		<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>
	<br><b style="font-size:1.3rem">Usage</b><br>		<br><b style="font-size:1.3rem">Usage</b><br>
−	<br>We ligased the <i>coaBC</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. <br>	+	<br>We ligased the <i>coaBC</i> fragment and <i>pspA</i> promoter on the pUC expression vector and transformed it into DH5α to complete construction of the plasmid. <br>
	<br><b style="font-size:1.3rem">Characterization</b><br>		<br><b style="font-size:1.3rem">Characterization</b><br>

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Revision as of 03:09, 22 October 2021

PpspA-CoaBC

Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.

Biology

Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.

Fig.1 Taurine pathways in E. coli

Usage

We ligased the coaBC fragment and pspA promoter on the pUC expression vector and transformed it into DH5α to complete construction of the plasmid.

Characterization

Fig. 2. Colony PCR confirmation of the construction

Reference

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features