Difference between revisions of "Part:BBa K4060100"
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===Usage and Biology===
 
===Usage and Biology===
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                        <div class="img">
 
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<img src="https://2021.igem.org/wiki/images/a/a7/T--NYCU-Taipei--R1.png" alt="">
 
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<div class="description"><b></b>Fig.2 Agarose electrophoresis in order to verify Pre A1.
 
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On the right is the expected results and on the left is the obtained confirmation agarose gel.
 
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Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder.
 
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Lane 2, Pre A1 digested with BamHI EcoRI PstI.
 
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Lane 3, Pre A1 digested with NheI EcoRI PstI.
 
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Lane 4, Pre A1 digested with EcoRI PstI.
 
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</div>
 
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</div>
 
  
 
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Revision as of 03:08, 22 October 2021


pBad ligated with RBS

pBad promote TetR expression

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]