Difference between revisions of "Part:BBa K4060100"
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | <div class="img"> | ||
| + | <img src="https://2021.igem.org/wiki/images/a/a7/T--NYCU-Taipei--R1.png" alt=""> | ||
| + | <div class="description"><b></b>Fig.2 Agarose electrophoresis in order to verify Pre A1. | ||
| + | On the right is the expected results and on the left is the obtained confirmation agarose gel. | ||
| + | Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder. | ||
| + | Lane 2, Pre A1 digested with BamHI EcoRI PstI. | ||
| + | Lane 3, Pre A1 digested with NheI EcoRI PstI. | ||
| + | Lane 4, Pre A1 digested with EcoRI PstI. | ||
| + | </div> | ||
| + | </div> | ||
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Revision as of 03:03, 22 October 2021
pBad ligated with RBS
pBad promote TetR expression
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]