Difference between revisions of "Part:BBa K3724014"

Revision as of 03:02, 22 October 2021

SO_3468+ribBA+ribE cluster

This gene cluster consists of the genes SO_3468, rib ba and ribe which are genes that encode proteins involved in the riboflavin biosynthesis pathway in Shewanella oneidensis MR-1. This gene cluster makes up a component of the riboflavin synthesis gene cluster (BBa_K3724015).

Usage and Biology

The the riboflavin synthase alpha subunit (SO_3468) which catalyzes the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil from two molecules of 6,7-dimethyl-8-ribityllumazine, the GTP cyclohydrolase-2 (ribA), 3,4-dihydroxy-2-butanone-4-phosphate synthase (ribB) and riboflavin synthase beta subunit (RibE). The latter three proteins catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate , formate and pyrophosphate, catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate and catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate, respectively. Sequence and Features