Difference between revisions of "Part:BBa K3777003"

 
 
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an antisense RNA strand which is partially complementary to 3WJdB(BBa_K3777000)
 
an antisense RNA strand which is partially complementary to 3WJdB(BBa_K3777000)
  
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https://static.igem.org/mediawiki/parts/thumb/3/30/T--OUC-China--result--V.png/800px-T--OUC-China--result--V.png
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<br>Verifying the strand replacement reaction. Promoter J23104 is much stronger than J23110. TetR-T7(tetO)-3WJdB has no KB2, so it is set as a negative control.
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<br>The result of this experiment proved that it’s valid to repress the fluorescence in vivo, which strongly support the feasibility of the ‘improved circuit’.
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<br>
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<br>Reference：Alam Khalid K,Tawiah Kwaku D,Lichte Matthew F,Porciani David,Burke Donald H. A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.[J]. ACS synthetic biology,2017,6(9)
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 02:58, 22 October 2021


kleptamers

an antisense RNA strand which is partially complementary to 3WJdB(BBa_K3777000)

800px-T--OUC-China--result--V.png
Verifying the strand replacement reaction. Promoter J23104 is much stronger than J23110. TetR-T7(tetO)-3WJdB has no KB2, so it is set as a negative control.
The result of this experiment proved that it’s valid to repress the fluorescence in vivo, which strongly support the feasibility of the ‘improved circuit’.

Reference：Alam Khalid K,Tawiah Kwaku D,Lichte Matthew F,Porciani David,Burke Donald H. A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.[J]. ACS synthetic biology,2017,6(9)
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]