Difference between revisions of "Part:BBa K3714002"
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<center>[[File:K3714002-1.png]]</center>
 
<center>[[File:K3714002-1.png]]</center>
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<center><b>Figure.3 | Verification of cleavage property of aptazyme. </b> T7 transcription products were loaded on a denatured PAGE to determine its cleave fraction.</center>
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<center><b>Figure.2 | Verification of cleavage property of aptazyme. </b> T7 transcription products were loaded on a denatured PAGE to determine its cleave fraction.</center>
  
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<center>[[File:K3714002-2.png]]</center>
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<center>[[File:K3714002.png]]</center>
 
<center><b>Figure.3 | Verification of lateral flow assay. </b> T7 transcription product with a target concentration gradient were used as input of LFA.</center>
 
<center><b>Figure.3 | Verification of lateral flow assay. </b> T7 transcription product with a target concentration gradient were used as input of LFA.</center>
  
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<center>[[File:K3714002-2.png]]</center>
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<center><b>Figure.4 | Performance of lateral flow assay. </b> The strips in Figure.3 were analyzed by ImageJ, and we used a two-phased linear regression to fit the results.</center>
  
 
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Revision as of 02:56, 22 October 2021


Biosensor gard-337 for lateral flow assay

The template of aptazyme gard-337 with two probes (binding BBa_K3714007 and BBa_K3714008) on both terminal for lateral flow assay (LFA). It could be used as a biosensor of gardiquimod to verify our design on a colloidal gold based detection platform (Figure.1).

K3714002-0.png
Figure.1 | Design of lateral flow assay. T7 transcription products with or without certain molecule are used as input of LFA. When the certain molecule exists, positive signal could be seen on test line. T line, test line. C line, control line.
K3714002-1.png
Figure.2 | Verification of cleavage property of aptazyme. T7 transcription products were loaded on a denatured PAGE to determine its cleave fraction.
K3714002.png
Figure.3 | Verification of lateral flow assay. T7 transcription product with a target concentration gradient were used as input of LFA.


K3714002-2.png
Figure.4 | Performance of lateral flow assay. The strips in Figure.3 were analyzed by ImageJ, and we used a two-phased linear regression to fit the results.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]