Difference between revisions of "Part:BBa K3714002"

Revision as of 02:53, 22 October 2021

Biosensor gard-337 for lateral flow assay

The template of aptazyme gard-337 with two probes (binding BBa_K3714007 and BBa_K3714008) on both terminal for lateral flow assay (LFA). It could be used as a biosensor of gardiquimod to verify our design on a colloidal gold based detection platform (Figure.1).

Figure.1 | Design of lateral flow assay. T7 transcription products with or without certain molecule are used as input of LFA. When the certain molecule exists, positive signal could be seen on test line. T line, test line. C line, control line.

Figure.3 | Verification of cleavage property of aptazyme. T7 transcription products were loaded on a denatured PAGE to determine its cleave fraction.

Figure.3 | Verification of lateral flow assay. T7 transcription product with a target concentration gradient were used as input of LFA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <center>[[File:K3714002-0.png]]</center>
 <center><b>Figure.1 | Design of lateral flow assay. </b> T7 transcription products with or without certain molecule are used as input of LFA. When the certain molecule exists, positive signal could be seen on test line. T line, test line. C line, control line.</center>
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <center>[[File:K3714002-1.png]]</center>
 <center><b>Figure.3 | Verification of cleavage property of aptazyme. </b> T7 transcription products were loaded on a denatured PAGE to determine its cleave fraction.</center>
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+<!-- Add more about the biology of this part here
+===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>