Difference between revisions of "Part:BBa K3898152:Design"

 
 
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===Design Notes===
 
===Design Notes===
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1
 
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This sequence was optimized for <i>E. coli BL21</i> using the Codon Optimization Tool from the genscript website.
  
 
===Source===
 
===Source===
  
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1
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R0184, J61100, K3898001, K3898003, B0012
  
 
===References===
 
===References===

Latest revision as of 02:44, 22 October 2021


RIDD-PETase：optimized PETase with RIDD (comparing with BBa_K2010999)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 360
    Illegal AgeI site found at 74
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimized for E. coli BL21 using the Codon Optimization Tool from the genscript website.

Source

R0184, J61100, K3898001, K3898003, B0012

References