Difference between revisions of "Part:BBa K4060506:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | We use PCR technique to clone the gene fragment from template pKA-207I10(Plasmid#79845) from Addgene. We add restriction enzyme cutting site that according to igem part assembly protocol RFC#10 to both sides of the gene fragment, then using these restriction enzyme cutting site to insert the gene fragment into vector pSB1C3. | |
Latest revision as of 02:39, 22 October 2021
QPAS1 gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 151
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use PCR technique to clone the gene fragment from template pKA-207I10(Plasmid#79845) from Addgene. We add restriction enzyme cutting site that according to igem part assembly protocol RFC#10 to both sides of the gene fragment, then using these restriction enzyme cutting site to insert the gene fragment into vector pSB1C3.
Source
This part is cloned from Addgene plasmid pKA-207I10.