Difference between revisions of "Part:BBa K3747201:Experience"

 
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===Applications of BBa_K3747201===
 
===Applications of BBa_K3747201===
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This biobrick can be used to make an AHL reporter strain. The strain produces LuxR which binds to AHL. Upon high cell densities, the concentration of AHL increases, activating the lux pR promoter. When activated, GFP is produced which can be measured using plate reader experiments. 
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[[File:T--Wageningen_UR--wetlab_1.png|thumb|center|<b>Figure 1</b> GFP is under control of the lux pR promoter which is activated by the luxR-AHL complex. LuxR is constitutively expressed. In this construct, no AHL is produced, so the lux pR promoter should only be induced when AHL is present in the medium. ]]
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===User Reviews===
 
===User Reviews===

Revision as of 02:33, 22 October 2021


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Please enter how you used this part and how it worked out.

Applications of BBa_K3747201

This biobrick can be used to make an AHL reporter strain. The strain produces LuxR which binds to AHL. Upon high cell densities, the concentration of AHL increases, activating the lux pR promoter. When activated, GFP is produced which can be measured using plate reader experiments.

Figure 1 GFP is under control of the lux pR promoter which is activated by the luxR-AHL complex. LuxR is constitutively expressed. In this construct, no AHL is produced, so the lux pR promoter should only be induced when AHL is present in the medium.


User Reviews

UNIQe2d9705a1fa5409d-partinfo-00000000-QINU UNIQe2d9705a1fa5409d-partinfo-00000001-QINU