Difference between revisions of "Part:BBa K3714010"
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<partinfo>BBa_K3714010 short</partinfo> | <partinfo>BBa_K3714010 short</partinfo> | ||
− | To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor. | + | To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor. <br/> |
− | This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1). | + | This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1). <br/> |
+ | The stop oligo significantly in | ||
+ | <br/> | ||
+ | <center>[[File:K3714016.png]]</center> | ||
+ | <center><b>Figure.1 | Verification of temporarily inhibition and disinhibition by oligonucleotides. </b>The aptazyme was produced by a T7 <i>in vitro</i> transcription system. The product was run on a denatured PAGE. Gardiquimod is an inhibitor of the cleavage activity and "-gardiquimod" is a control group. "stop" and "start" are two oligonucleotides designed to control the activity of the aptazyme/</center> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3714010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3714010 SequenceAndFeatures</partinfo> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3714010 parameters</partinfo> | <partinfo>BBa_K3714010 parameters</partinfo> | ||
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Revision as of 02:24, 22 October 2021
Improved gard-337 for lateral flow assay
To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor.
This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1).
The stop oligo significantly in
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]